Why is nmr insensitive

Nowadays, its versatility allows the three-dimensional structure determination of biological macromolecules, the characterisation of protein dynamics, kinetic and thermodynamic information on protein folding, as well as on molecular interactions. The interface between methodological advancements in NMR spectroscopy, bio-computing and structural biology provides a comprehensive approach to address biological problems. The major limitation of NMR arises from the increase of transverse relaxation rates which prevents the investigation of molecules with large molecular weights.

In the last few years, cross correlation rates have been shown to be a way to investigate molecules with large molecular masses. This project has developed new approaches based on the direct-detection of 13C to study fast relaxing nucleus systems by NMR. The new methodologies here tuned up has being applied both to paramagnetic metalloproteins, Calbindin D9K publications 1, 2 , and to medium size metalloproteins, CutA1 publication 3 , but it has being also proved to work on diamagnetic metalloproteins, c2 SOD publication 4.

In this project, we have accomplished both methodological aims: pushing NMR frontiers in the detection of resonance belonging to fast relaxation nuclei publications 1 and 3 , developing new pulse sequences to obtain new structural constraints publication 2 ; and also biological objectives publications 3, 4 and 5.

A comparison of 13 C nuclear magnetic resonance and 14C tracer studies of hepatic metabolism. Determination of the intracellular sodium concentration in perfused mouse liver by 31 P and 23 Na magnetic resonance spectroscopy.

Magn Reson Med 39 : — Magn Reson Med 20 : 17 — Alterations in liver ATP homeostasis in human nonalcoholic steatohepatitis—A pilot study. JAMA : — Serial 1 H-NMR spectroscopy study of metabolic impairment in primates chronically treated with the succinate dehydrogenase inhibitor 3-nitropropionic acid.

Neurobiol Dis 6 : — Early N-acetylaspartate depletion is a marker of neuronal dysfunction in rats and primates chronically treated with the mitochondrial toxin 3-nitropropionic acid. J Cerebr Blood Flow Metab 20 : — Mesial motor areas in self-initiated versus externally triggered movements examined with fMRI: Effect of movement type and rate. J Neurophysiol 81 : — NMR studies of the effect of hyperglycemia on intracellular cations in rat kidney.

Biochim Biophys Acta : — Multisection proton MR spectroscopic imaging of the brain. Oxford : Clarendon Press. Ernst T Hennig J. Improved water suppression for localized in vivo 1 H spectroscopy. J Magn Reson B : — New York : Academic Press. Practical NMR Imaging. Oxford : IRL Press. Differentiation between renal cortex and medulla in the response to hypotension using localized 31 P magnetic resonance spectroscopy.

Transplantation 48 : — Gadian D. A versatile oxygenator and perfusion system for magnetic resonance studies. Biotech Bioeng 49 : — Noninvasive detection of elevated glutathione levels in MCF-7 cells resistant to 4-hydroperoxycyclophosphamide.

Cancer Res 55 : — Phosphorus NMR studies on perfused heart. Biochem Biophys Res Commun 74 : — Gilles RJ. NMR in Physiology and Biomedicine. San Diego : Academic Press. Representation of the temporal envelope of sounds in the human brain. J Neurophysiol 84 : — Dev Neurosci 20 : — Steady-state cerebral glucose concentrations and transport in the human brain.

J Neurochem 70 : — Ren Physiol Biochem 12 : — Vascular smooth muscle glycogen metabolism studied by 13 C-NMR. J Vase Res 32 : — Harris RK. Nuclear Resonance Spectroscopy. London : Longmans. Herman GT. Computer Science and Applied Mathematics. Radiographic thin-section image of the human wrist by nuclear magnetic resonance. Detection of regional ischemia in perfused beating hearts by phosphorus nuclear magnetic resonance.

Biochem Biophys Res Commun 75 : — Observation of tissue metabolites using 31P nuclear magnetic resonance. Oxidative glucose metabolism in rat brain during single forepaw stimulation: A spatially localized 'H[13C] nuclear magnetic resonance study. J Cereb Blood Flow Metab 17 : — Phorphorus nuclear magnetic resonance of perfused working rat hearts.

Substrate selection in the isolated working rat heart: Effects of reperfusion, afterload andconcentration. Basic Res Cardiol 90 : — MR imaging of laser-tissue interactions. Imaging of current density and current pathways in rabbit brain during transcranial electrostimulation.

In vivo detection of applied electric currents by magnetic resonance imaging. Magn Reson Imaging 7 : 89 — Metabolic control analysis of insulin-stimulated glucose disposal in rat skeletal muscle.

In vivo NMR investigation of intramuscular glucose metabolism in conscious rats. Receptor-targeted cotransport of DNA and magnetic resonance constrast agents. Chem Biol 2 : — Proton MR spectroscopy of delayed cerebral radiation in monkeys and humans after brachytherapy. AJNR 18 : — Analysis of 23Na NMR spectra from isolated perfused hearts. Magn Reson Med 18 : 15 — In vivo monitoring of changes in 5-fluorouracil metabolism induced by methotrexate measured by 19 FNMR spectroscopy.

Magn Reson Med 19 : — Citrate alterations in primary and metastatic human prostatic adenocarcinomas: 1 H magnetic resonance spectroscopy and biochemical study. Magn Reson Med 29 : — Kushmerick MJ. Skeletal muscle: A paradigm for testing principles of bioenergetics.

J Bioenerg Biomembr 27 : — Pathophysiology and pathogenesis of contractile failure in stunned myocardium. Jpn Circ J 55 : — The time course of myocardial glycogenolysis stimulated by glucagon. J Mol Cell Cardiol 25 : — Nonglucose substrates increase glycogen synthesis in vivo in dog heart.

Regulation of glycogen metabolism in canine myocardium: Effects of insulin and epinephrine in vivo. Lauterbur PC. Image formation by induced local interactions: Examples employing nuclear magnetic resonance. Effects of hypoxia on energy state and pH in resting pulmonary and femoral arterial smooth muscles.

Lebihan D , ed. Diffusion and Perfusion Magnetic Resonance Imaging. Applications to Functional MRI. New York : Raven Press. Phosphorus and carbon nuclear magnetic resonance study of glucose and xylose metabolism in agarose-immobilized Candida tropicalis. Appl Environ Microbiol 61 : — In vivo visualization of gene expression using magnetic resonance imaging. Nature Biotech 18 : — Analysis of tricarboxylic acid cycle of the heart using 13 C isotope isomers. Neurochem Res 21 : — Mansfield P Morris PG.

NMR Imaging in Biomedicine. Intracellular free calcium concentration measured with NMR spectroscopy in intact ferret hearts. J Cereb Blood Flow Metab 15 : 12 — Characterizing the hemodynamic response: Effects of presentation rate, sampling procedure, and the possibility of ordering brain activity based on relative timing. Neuroimage 11 Pt 1 : — Irreversible regional cerebral ischemia: Serial MR imaging and proton MR spectroscopy in a nonhuman primate model.

AJNR 14 : — Determination of intracellular pH by 31 P magnetic resonance. NMR Biomed 2 : — Early detection of regional cerebral-ischemia in cats?

Comparison of diffusion-weighted and T2-weighted MRI and spectroscopy. Magn Reson Med 14 : — Tissue temperature monitoring with multiple gradient-echo imaging sequences. J Magn Reson Imaging 8 : — Narayan P Kurhanewicz J. Magnetic resonance spectroscopy in prostate disease: Diagnostic possibilities and future developments.

Prostate Suppl 4 : 43 — Magn Reson Med 11 : — Hum Brain Mapp 6 : — Development of a bioartificial pancreas: II. Effects of oxygen on long- term entrapped betaTC3 cell cultures. Biotechnol Bioeng 66 : — Magnetic Resonance Imaging. Philadelphia : WB Saunders. Phelps ME. PET: The merging of biology and imaging into molecular imaging. J Nucl Med 41 : — Positron emission tomography provides molecular imaging of biological processes. Intracellular volume and apparent diffusion constants of perfused cancer cell cultures, as measured by NMR.

Magn Reson Med 37 : — Developmental adaptations in cytosolic phosphate content and pH regulation in the sheep heart in vivo. J Clin Invest 86 : — Influence of the pH of cardioplegic solutions on cellular energy metabolism and hydrogen ion flux during neonatal hypothermic circulatory arrest and reperfusion: A dynamic 31 P nuclear magnetic resonance study in a pig model. J Thorac Cardiovasc Surg : — In vivo 19 F NMR spectroscopy of the antimetabolite 5-fluorouracil and its analogues.

An assessment of drug metabolism. Biochem Pharmacol 39 : — Resonance absorption by nuclear magnetic moments in a solid. Phys Rev 69 : 37 — Impairment of cardiac function and energetics in experimental renal failure. J Clin Invest 92 : — The neural bases of strategy and skill in sentence-picture verification. Cog Psychol 40 : — NMR Biomed 8 : 49 — Schlicter CP.

Principles of Magnetic Resonance. New York : Springer-Verlag. The cerebellar cognitive affective syndrome. Brain Pt 4 : — Energetics of acute pressure overload of the porcine right ventricle. In vivo 3 IP nuclear magnetic resonance. J Clin Invest 89 : — In vivo 31 PNMR spectroscopy of right ventricle in pigs. Histological validation of myocardial microstructure obtained from diffusion tensor magnetic resonance imaging.

Sequin U Scott AI. Carbon as a label in biosynthetic studies. Science : — NMR Biomed 5 : — In vivo 13 C NMR measurements of cerebral glutamine synthesis as evidence for glutamate-glutamine cycling. Stoichiometric coupling of brain glucose metabolism and glutamatergic neuronal activity. Functional energy metabolism: In vivo 13 C-NMR spectroscopy evidence for coupling of cerebral glucose consumption and glutamatergic neuronalactivity. Method for intracellular magnetic labeling of human mononuclear cells using approved iron contrast agents.

Magn Reson Imaging 17 : — ICAM-1 expression in autoimmune encephalitis visualized using magnetic resonance imaging. J Neuroimmunol : 1 — 9. New aspects of lactate metabolism: IGF-I and insulin regulate mitochondrial function in cultured brain cells during normoxia and hypoxia. Dev Neurosci 18 : — Acta Physiol Scand : — Neural structures associated with recognition of facial expressions of basic emotions. Louis : Mosby. Role of increased cytosolic free calcium concentration in myocardial ischemic injury.

Basic Res Cardiol 88 : — Robertson , Lysbeth H. Antonides , Nicolas Gilbert , Sophie L. Benjamin , Stuart K. Langley , Lindsey J. Munro , Oliver B. Sutcliffe , Ryan E. ChemistryOpen , 8 12 , Yurkovskaya , Konstantin L. Lindale , Shannon L. Eriksson , Christian P. Tanner , Zijian Zhou , Johannes F. Chekmenev , Thomas Theis , Warren S.

Unveiling coherently driven hyperpolarization dynamics in signal amplification by reversible exchange. Pravdivtsev , Bryce Kidd , C. Zacharias , Eduard Y.

Angewandte Chemie , 35 , Angewandte Chemie International Edition , 57 35 , Coffey , Roman V. Shchepin , Bibo Feng , Raul D. Colon , Ken Wilkens , Kevin W. Waddell , Eduard Y. A pulse programmable parahydrogen polarizer using a tunable electromagnet and dual channel NMR spectrometer.

Israel Journal of Chemistry , 57 9 , Singlet order conversion and parahydrogen-induced hyperpolarization of 13C nuclei in near-equivalent spin systems. Symmetry constraints on spin dynamics: Application to hyperpolarized NMR. Yurkovskaya , Nikita N. Exploiting adiabatically switched RF-field for manipulating spin hyperpolarization induced by para hydrogen. The Journal of Chemical Physics , 23 , Pravdivtsev , K.

Ivanov , A. Yurkovskaya , H. The author recommends the textbook Spin Dynamics by Malcolm H. Levitt as a companion for using Insensitive. Also available for the iPhone.